scRNA-seq pipeline 2021.1

pip install jupyterlab
pip install jupyter_contrib_nbextensions
jupyter contrib nbextension install –user
pip install jupyter_nbextensions_configurator
jupyter nbextensions_configurator enable –user

pip install anndata
pip install scanpy
pip install scvelo
pip install episcanpy
conda install -c conda-forge r-base=4.0.3
conda install -c conda-forge libgit2     or brew install libgit2    on mac
conda install -c conda-forge r-cairo
conda install -c conda-forge r-hdf5r
install.packages(c(“devtools”,”tidyverse”))

devtools::install_git(“https://gitee.com/chansigit/seurat.git”)
devtools::install_git(“https://gitee.com/chansigit/uwot.git”)
devtools::install_git(“https://gitee.com/chansigit/liger.git”)
devtools::install_git(“https://gitee.com/chansigit/seurat-wrappers.git”)

if (!requireNamespace(“BiocManager”, quietly = TRUE))
install.packages(“BiocManager”)
BiocManager::install(version = “3.12”)
BiocManager::install(c(
‘BiocGenerics’, ‘DelayedArray’, ‘DelayedMatrixStats’,
‘limma’, ‘S4Vectors’, ‘SingleCellExperiment’,’multtest’,
“SingleCellExperiment”,”GenomicRanges”,”scRNAseq”,
“Rhdf5lib”,”pcaMethods”,”DropletUtils”,”scater”,
“SingleR”,”geneplotter”,”AUCell”,”GSVA”,”ComplexHeatmap”,
“AnnotationHub”,’SummarizedExperiment’, ‘batchelor’, ‘Matrix.utils’,
“clusterProfiler”,”ChIPseeker”,”ChIPpeakAnno”))

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